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001009s1999 njua ob 001 0 eng d |
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|a 0585246211 (electronic bk.)
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|a 9780585246215 (electronic bk.)
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|z 0896037312 (alk. paper)
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|a (OCoLC)45729884
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|a (OCoLC)45729884
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|a N$T
|b eng
|c N$T
|d OCL
|d OCLCQ
|d MUQ
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|d OCLCQ
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|a HCDD
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|a E-Book RC691.4
|b .V36 1999
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1 |
0 |
|a WG 500
|b V33128 1999
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| 072 |
|
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|a MED
|x 010000
|2 bisacsh
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| 245 |
0 |
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|a Vascular disease
|h [electronic resource] :
|b molecular biology and gene therapy protocols /
|c edited by Andrew H. Baker.
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| 260 |
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|a Totowa, N.J. :
|b Humana Press,
|c c1999.
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| 300 |
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|a 1 online resource (xiv, 441 p.) :
|b ill.
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| 490 |
1 |
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|a Methods in molecular medicine ;
|v 30
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| 490 |
1 |
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|a Ebsco e-book purchased
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| 504 |
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|a Includes bibliographical references and index.
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| 588 |
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|a Description based on print version record.
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| 505 |
0 |
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|a Pt. I. Genetics in Vascular Disease. 1. Detection of Mutations and DNA Polymorphisms in Genes Involved in Cardiovascular Diseases by Polymerase Chain Reaction -- Single-Strand Conformation Polymorphism Analysis / Shu Ye and Adriano M. Henney. 2. Analysis of Genetic Variants in Cardiovascular Risk Genes by Heteroduplex Analysis / Ana Cenarro, Fernando Civeira and Miguel Pocovi. 3. Radiation Hybrid (RH) Mapping of Human Smooth Muscle-Restricted Genes / Joseph M. Miano, Emilio Garcia and Ralf Krahe -- Pt. II. Isolation of Genes Expressed in Vascular Tissue. 4. Efficient Extraction of RNA from Vascular Tissue / Catherine F. Townsend, Christopher M. H. Newman and Sheila E. Francis. 5. Preparation of cDNA Libraries from Vascular Cells / Mark E. Lieb and Mark B. Taubman. 6. Screening cDNA Libraries Using Partial Probes to Isolate Full-Length cDNAs from Vascular Cells / Csilla Csortos, Virginie Lazar and Joe G. N. Garcia. 7. Cloning Full-Length cDNAs from Vascular Tissues and Cells by Rapid Amplification of cDNA Ends (RACE) and RT-PCR / Rong-Fong Shen. 8. Use of cDNA Representational Difference Analysis to Identify Disease-Specific Genes in Human Atherosclerotic Plaques / Kerry Tyson and Catherine Shanahan. 9. The Use of Differential mRNA Display (DDRT-PCR) to Identify Genes Differentially Expressed in Normal and Diseased Vascular Cells / Paul J. Adam. 10. Identification of Novel Protein Kinases in Vascular Cells / Ian Zachary, Spiros Servos and Barbara Herren -- Pt. III. Methods for Mapping Transcriptional Start Sites and Measurement of Promoter Activity in Vascular Cells. 11. Primer Extension Analysis to Map Transcription Start Sites of Vascular Genes / Yutaka Kitami and Kunio Hiwada. 12. Use of Liposome-Mediated DNA Transfection to Determine Promoter Activity in Smooth Muscle Cells / Rosalind P. Fabunmi. 13. Nuclear Run-On Assay to Study Gene Transcription in Vascular Cells / Ulrich Laufs and James K. Liao. 14. Electromobility Shift Analysis (EMSA) Applied to the Study of NF-kappa B Binding Interactions in Vascular Smooth Muscle Cells / Todd Bourcier -- Pt. IV. Molecular Analysis of mRNA Expression in Vascular Cells. 15. Measurement of Gene Expression in the Vascular Wall by Reverse Transcription-Polymerase Chain Reaction (RT-PCR) Analysis / Joachim Fruebis. 16. Northern Blot Analysis to Quantify Gene Expression in Vascular Diseases / Mingyi Chen and Tatsuya Sawamura. 17. Localization of Gene Expression in Human Atherosclerotic Lesions by In Situ Hybridization / Lee D. K. Buttery and Julia M. Polak. 18. RNase Protection Assays for Quantitation of Gene Expression in Vascular Tissue / Nengyu Yang, Shuli Wang and James E. Faber -- Pt. V. Molecular Methods to Study Apoptosis and Phenotypic Changes in Vascular Cells and Tissue. 19. Detection of Apoptosis in Atherosclerosis and Restenosis by Terminal dUTP Nick-End Labeling (TUNEL) / Mark Kockx, Johannes Muhring and Michiel Knaapen. 20. In Vitro Detection of Apoptosis in Isolated Vascular Cells / Shiu-Wan Chan and Martin R. Bennett. 21. Molecular Assessment of the Phenotypic Changes Associated with Smooth Muscle Cells Using Two-Dimension Electrophoresis and Microsequence Analysis / Rachel Johnatty, Giulio Gabbiani and Pascal Neuville -- Pt. VI. In Vitro Methods to Express Foreign Genes in Vascular Cells. 22. Simple Methods for Preparing Recombinant Adenoviruses for High-Efficiency Transduction of Vascular Cells / Stuart A. Nicklin and Andrew H. Baker. 23. Generation of Recombinant Adeno-Associated Viruses for Delivery of Genes into Vascular Cells / Carmel M. Lynch. 24. Hemagglutinating Virus of Japan Liposome-Mediated Gene Delivery to Vascular Cells / Yoshikazu Yonemitsu and Yasufumi Kaneda. 25. High-Efficiency and Low-Toxicity Adenovirus-Assisted Endothelial Transfection / Takamune Takahashi, Keiko Takahashi and Thomas O. Daniel. 26. Gene Transfer in Vascular Cells Using an Engineered Na-H Exchanger (NHE1) as a Selectable Market / Jacques Pouyssegur and Daniele Roux. 27. Use of Retroviruses to Express Exogenous Genes in Vascular Smooth Muscle Cells / Trevor D. Littlewood and Stella A. Pelengaris. 28. Embryonal Stem (ES) Cell-Derived Macrophages: A Cellular System that Facilitates the Genetic Dissection of Macrophage Function / Kathryn J. Moore and Mason W. Freeman. 29. Delivery of Recombinant Adenoviruses to Human Saphenous Vein / Sarah J. George and Andrew H. Baker -- Pt. VII. In Vivo Vascular Gene Transfer Protocols. 30. An In Vivo Angiogenesis Assay to Study Positive and Negative Regulators of Neovascularization / Maurizio C. Capogrossi and Antonino Passaniti. 31. Efficient Liposome-Mediated Gene Transfer to Rabbit Carotid Arteries in Vivo / Michael-Christopher Keogh and Daxin Chen. 32. Gene Delivery to Rabbit Arteries Using the Collar Model / Mikko O. Hiltunen, Mikko P. Turunen and Seppo Yla-Herttuala. 33. Delivery of Antisense Oligonucleotides to the Vascular Wall / Cathy M. Holt, Julian Gunn and Darren Lambert / [et al.]. 34. Local Gene Delivery of Recombinant Adenoviruses to the Rat Carotid Artery In Vivo / Clare M. Dollery and Jean R. McEwan.
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| 590 |
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|a Loaded electronically.
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| 590 |
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|a Electronic access restricted to members of the Holy Cross Community.
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| 590 |
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|a This was purchased from Ebsco with a single user license.
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| 650 |
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0 |
|a Blood-vessels
|x Diseases
|x Molecular aspects
|v Laboratory manuals.
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| 650 |
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0 |
|a Blood-vessels
|x Diseases
|x Gene therapy
|v Laboratory manuals.
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| 650 |
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0 |
|a Blood-vessels
|x Diseases
|x Genetic aspects
|v Laboratory manuals.
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| 655 |
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7 |
|a Electronic books.
|2 local
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| 690 |
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|a Electronic resources (E-books)
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| 700 |
1 |
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|a Baker, Andrew H.
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| 830 |
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0 |
|a Methods in molecular medicine ;
|v 30.
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| 830 |
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0 |
|a Ebsco e-book purchased.
|
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|u https://holycross.idm.oclc.org/login?auth=cas&url=https://search.ebscohost.com/login.aspx?direct=true&scope=site&db=nlebk&db=nlabk&AN=28041
|3 Click to view e-book
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