Hemostasis and thrombosis protocols

Hemostasis and thrombosis protocols edited by David J. Perry and K. John Pasi.

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Bibliographic Details
Other Authors: Perry, David (David J.), Pasi, K. John
Format: eBook
Language:English
Published: Totowa, N.J. : Humana Press, c1999.
Series:Methods in molecular medicine ; 31.
Ebsco e-book purchased.
Subjects:
Blood coagulation disorders > Molecular aspects > Laboratory manuals.
Blood > Coagulation > Laboratory manuals.
Thrombosis > Laboratory manuals.
Electronic resources (E-books)
Online Access:Click to view e-book
Holy Cross Note:Loaded electronically.
Electronic access restricted to members of the Holy Cross Community.
This was purchased from Ebsco with a single user license.
Table of Contents:
  • pt. 1. Introduction
  • Hemostasis: components and processes / K. John Pasi
  • pt. 2. Basic techniques
  • Isolation of DNA and RNA / David J. Perry
  • Amplification of DNA and RNA by PCR / David J. Perry
  • Direct sequencing of PCR products / David J. Perry
  • Solid-phase sequencing of biotinylated PCR products with streptavidin-coated magnetic beads / David J. Perry
  • Automated DNA sequencing / Helen L. Devereux
  • Detection of DNA by silver staining / David J. Perry
  • Promoter studies in hemostasis / Peter R. Winship
  • pt. 3. Methods of mutational analysis
  • Detection of mutations and polymorphisms in clotting factors by denaturing gradient gel electrophoresis / Rainer Schwaab
  • Screening for mutations in DNA by single-stranded conformation polymorphism (SSCP) analysis / David J. Perry
  • Screening for DNA heteroduplexes in the factor VII gene using ethylene glycol gel electrophoresis of solvent-treated 32P-labeled PCR products / Peter M. Baker
  • Detection of mutations causing hemophilia A using in vitro coupled transcription and translation system / Chike Ononye
  • Screening for mutations in the human antithrombin gene by Hydrolink D-5000 and MDE gel electrophoresis / David J. Perry
  • pt. 4. Methods for analyzing inherited/acquired disorders of hemostasis
  • Detection of mutations in hemophilia A patients by chemical cleavage of mismatch method / Naushin H. Waseem
  • Inversion mutation analysis in hemophilia A by restriction enzyme analysis and southern blotting / Chike Ononye
  • Hemophilia B mutational analysis / Peter M. Green
  • Screening for candidate mutations causing von Willebrand's Disease (vWD) / P. Vincent Jenkins
  • Use of intron 40 VNTR I in vWD gene tracking / Mohammed S. Enayat
  • Mutlimeric analysis of von Willebrand Factor (vWF) / Mohammed S. Enayat
  • Identification of mutations in the human factor VII gene / Peter M. Baker
  • Molecular analysis in factor XI deficiency / Karen M. Johnson
  • Mutational analysis in antithrombin deficiency / David J. Perry
  • Ectopic transcript analysis in human antithrombin deficiency / David J. Perry
  • Mutational analysis of the human protein C gene / Roger Luddington
  • Analysis of the protein S gene in protein S deficiency / Núria Sala
  • Screening for the G to A transition at position 20210 in the 3'-untranslated region (UTR) of the prothrombin gene / Karen P. Brown
  • Screening for the factor V leiden mutation / Karen P. Brown
  • Multiplex PCR for detection of the prothrombin 3' UTR (C20210A) polymorphism and the factor V leiden mutation / Gillian Mellars
  • Isoelectric focusing and immunodetection of plasma antithrombin / Martina Daly
  • Characterization of heparin binding variants of antithrombin by crossed immunoelectrophoresis in the presence of heparin / Martina Daly
  • The determination of amino acid sequence abnormalities in proteins by HPLC peptide analysis / David Williamson
  • pt. 5. Platelet and megakaryocyte analysis
  • Molecular biological identification and characterization of inherited platelet receptor disorders / Ramesh B. Basani
  • In vitro expansion of megakaryocytes from peripheral blood hematopoietic progenitors / Michael A. Thornton
  • Molecular biology studies with primary megakaryocytes / Yaping Shou.

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