Gas Chromatography/Mass Spectrometry

Gas Chromatography/Mass Spectrometry edited by Hans-Ferdinand Linskens, John F. Jackson.

Modern Methods of Plant Analysis When the handbook Modern Methods of Plant Analysis was first introduced in 1954 the considerations were: 1. the dependence of scientific progress in biology on the improvement of existing and the introduction of new methods; 2. the difficulty in finding many new anal...

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Bibliographic Details
Corporate Author: SpringerLink (Online service)
Other Authors: Linskens, Hans-Ferdinand (Editor), Jackson, John F. (Editor)
Format: eBook
Language:English
Published: Berlin, Heidelberg : Springer Berlin Heidelberg : Imprint: Springer, 1986.
Edition:1st ed. 1986.
Series:Molecular Methods of Plant Analysis, 3
Springer eBook Collection.
Subjects:
Plant science.
Botany.
Human physiology.
Biophysics.
Biological physics.
Chemistry, Physical and theoretical.
Biochemistry.
Electronic resources (E-books)
Online Access:Click to view e-book
Holy Cross Note:Loaded electronically.
Electronic access restricted to members of the Holy Cross Community.
Table of Contents:
  • The Use of Combined Gas Chromatography-Mass Spectrometry in the Analysis of Plant Growth Substances
  • 1 Introduction
  • 2 Identification by GC-MS
  • 3 Gibberellins
  • 4 Abscisic Acid and Related Compounds
  • 5 Brassinosteroids
  • References
  • Applications of Mass Spectrometry for the Examination of Pectic Polysaccharides
  • 1 Introduction
  • 2 Structural Analysis of Pectic Polysaccharides
  • 3 Determination of the Nature of the Glycosidic Linkages
  • 4 Separation and Identification of Partially Methylated Alditol Acetates
  • 5 Extensions of Methylation Analysis
  • 6 Sequencing of Sugar Residues in Pectins
  • 7 Sequencing of Pectic Polysaccharides by Partial Depolymerisation of Permethylated Derivatives
  • 8 Experimental
  • References
  • GC-MS Methods for Cyclic Nucleotides in Higher Plants and for Free High Unsaturated Fatty Acids in Oils
  • 1 Introduction
  • 2 Methods of GC-MS
  • 3 Adenosine-3?:5?-Monophosphate (cAMP) in Maize Seedlings (Zea mays)
  • 4 An Isotope Dilution GC-MS Spectrometric Assay for cAMP in Cultured Tobacco Tissue
  • 5 Stability of Cyclic Purine Nucleotides in the Presence of Hydrochloric Acid During Extraction
  • 6 Guanosine-3?:5?-Monophosphate (cGMP) in Maize Seedlings (Zea mays)
  • 7 GC-Separation of Synthetic cAMP and cGMP in a Mixture
  • 8 Cyclic Pyrimidine Nucleotides in Plants?
  • 9 Free High Unsaturated Fatty Acids in Oils
  • 10 Conclusions
  • References
  • GC-MS Methods for Lower Plant Glycolipid Fatty Acids
  • 1 Introduction
  • 2 Extraction of the Plant Material
  • 3 Separation of Glycolipids from the Total Lipid Extract
  • 4 Isolation of the Glycolipids
  • 5 Derivatization of the Glycolipid Fatty Acids for GC-MS
  • 6 GLC Instrumentation for Fatty Acid Analysis
  • 7 Column Selection for Fatty Acid GLC Analysis
  • 8 Interpretation of GC Data and Calculation of Results
  • 9 Fatty Acid Ester Structure Determination by GC-MS
  • 10 Abbreviations
  • References
  • Analysis of Phospholipid Molecular Species by Gas Chromatography and Coupled Gas Chromatography-Mass Spectrometry
  • 1 Introduction
  • 2 Lipid Preparation
  • 3 Formation of Derivatives for GC or GC-MS
  • 4 Gas Chromatography
  • 5 Mass Spectrometry
  • 6 Determination of Positional Distribution of Acyl Chains Using Phospholipase A2
  • 7 Conclusion
  • References
  • GC-MS of Plant Sterol Analysis
  • 1 Introduction
  • 2 Development of GC-MS Plant Sterol Analysis
  • 3 Operations Before GC-MS Sterol Analysis
  • 4 Characterization of Sterols
  • 5 Conclusion
  • References
  • GC-MS Methods for Terpenoids
  • 1 Introduction
  • 2 Isolation Methods
  • 3 Prefractionation and Ancillary Reactions
  • 4 Gas Chromatography
  • 5 Retention Data
  • 6 Mass Spectrometry
  • References
  • GC-MS of Auxins
  • 1 Introduction
  • 2 The Compounds Involved
  • 3 Reference Compounds
  • 4 Extraction
  • 5 Purification
  • 6 Columns for GC
  • 7 Injection Techniques
  • 8 Derivatisation
  • 9 Interface Between GC and MS
  • 10 Mass Spectrometer
  • 11 Data Systems
  • 12 Ionization
  • 13 GC-MS Strategy for Auxin Analysis
  • 14 Quantification
  • 15 The Internal Standard
  • 16 Experimental Procedure
  • 17 Conclusions
  • References
  • GC-MS Methods for the Quantitative Determination and Structural Characterization of Esters of Indole-3-Acetic Acid and myo-Inositol
  • 1 Introduction
  • 2 Quantitative Analysis and Identification of the IAA-Inositols
  • 2.2.1 A Quantitative Estimation of IAA-Inositol Using [3H]-IAA-myo-Inositol as an Internal Standard
  • 3 Qualitative Analysis of IAA-Inositols
  • 4 Conclusions
  • 5 Abbreviations
  • References
  • GC-MS Methods for Cytokinins and Metabolites
  • 1 Introduction
  • 2 Gas Chromatography (GC)
  • 3 Mass Spectrometry
  • 4 Applications of Mass Spectrometry in Cytokinin Analysis
  • 5 General Remarks and Conclusion
  • References
  • GC-MS Method for Volatile Flavor Components of Foods
  • 1 Introduction
  • 2 GC-MS Methods
  • 3 Volatile Flavor Components
  • References
  • GC-MS Methods for Tobacco Constituents
  • 1 Introduction
  • 2 Cembranoids and Their Degraded Compounds
  • 3 Labdanoids and Their Degraded Compounds
  • 4 Carotenoid-Degraded Compounds
  • 5 Sesquiterpenoids
  • 6 Terpenoid Glycosides
  • 7 Linked Scanning
  • References.

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