Capillary Electrophoresis: Principles and Practice

Capillary Electrophoresis: Principles and Practice by Reinhard Kuhn, Sabrina Hoffstetter-Kuhn.

Capillary electrophoresis (CE) is a brand-new analytical me- thod with the capability of solving many analytical separa- tion problems very fast and economically. This method gives new information about the investigated substances which can- not easily be obtained by other means. CE has become an es...

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Bibliographic Details
Main Authors: Kuhn, Reinhard (Author), Hoffstetter-Kuhn, Sabrina (Author)
Corporate Author: SpringerLink (Online service)
Format: eBook
Language:English
Published: Berlin, Heidelberg : Springer Berlin Heidelberg : Imprint: Springer, 1993.
Edition:1st ed. 1993.
Series:Springer Lab Manuals
Springer eBook Collection.
Subjects:
Analytical chemistry.
Biochemistry.
Food—Biotechnology.
Physical chemistry.
Electronic resources (E-books)
Online Access:Click to view e-book
Holy Cross Note:Loaded electronically.
Electronic access restricted to members of the Holy Cross Community.
Table of Contents:
  • 1 Introduction
  • 1.1 Safety Considerations
  • 1.2 History
  • 1.3 Nomenclature
  • 2 Basic Principles
  • 2.1 Basic Electrophoretic Separation Modes
  • 2.2 Set-up for Capillary Electrophoresis
  • 2.3 Theory of Electrophoretic Migration
  • 2.4 Determination of Effective Mobilitiy
  • 2.5 Electroosmosis
  • 2.6 Performance Criteria
  • 3 Factors Influencing Performance
  • 3.1 Fundamental Dispersive Effects
  • 3.2 Operational Parameters
  • 3.3 Electrolyte System
  • 4 Instrumentation
  • 4.1 Injection
  • 4.2 Detection
  • 4.3 Capillary Column
  • 4.4 Sample Collection
  • 4.5 Commercial Instruments
  • 5 Techniques
  • 5.1 Capillary Zone Electrophoresis
  • 5.2 Capillary Gel Electrophoresis
  • 5.3 Micellar Electrokinetic Chromatography
  • 5.4 Capillary Isotachophoresis
  • 5.5 Capillary Isoelectric Focusing
  • 5.6 Electrochromatography
  • 5.7 Hyphenated Techniques
  • 5.8 Special Techniques
  • 6 Qualitative and Quantitative Analysis
  • 6.1 General Aspects
  • 6.2 Influence of Injection
  • 6.3 Method Validation
  • 7 Applications
  • 7.1 Small Ions
  • 7.2 Sulphonates and Alkylsulphates
  • 7.3 Drugs and Natural Products
  • 7.4 Neutral Substances
  • 7.5 Herbicides
  • 7.6 Amino Acids, Peptides and Proteins
  • 7.7 Carbohydrates and Their Derivatives
  • 7.8 Nucleotides, Oligonucleotides and Nucleic Acids
  • 7.9 Chiral Molecules
  • 7.10 Complex Samples
  • 8 Appendix 331
  • 8.1 Buffer Tables
  • 8.2 Derivatization Procedures
  • 8.3 Glossary
  • 8.4 Manufacturers’ Directory
  • 8.5 Further Recommended Reading
  • References.

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