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|a 9783662001448
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|a 10.1007/978-3-662-00144-8
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|a Nonradioactive Labeling and Detection of Biomolecules
|h [electronic resource] /
|c edited by Christoph Kessler.
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|a 1st ed. 1992.
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|a Berlin, Heidelberg :
|b Springer Berlin Heidelberg :
|b Imprint: Springer,
|c 1992.
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|a XXIII, 436 p.
|b online resource.
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|a text
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|a Springer Lab Manuals
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|a Springer eBook Collection
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|a 1 General Aspects of Nonradioactive Labeling and Detection -- 1.1 Introduction -- 1.2 The Concept of Nonradioactive Bioanalytics. -- 1.3 Direct and Indirect Labeling and Detection Systems -- 1.4 Labeling Methods -- 1.5 Detection Methods -- 1.6 Guide of the Use of Information in this Book -- I Standard Nonradioactive Labeling and Detection Systems -- 2 Overview of Nonradioactive Labeling Systems -- 3 The Digoxigenin: Anti-Digoxigenin (DIG) System -- 4 The Biotin System -- 5 In Vivo Labeling of DNA Probes with 5-BrdU -- 6 The Sulfone System -- 7 Colloidal Gold as a Marker in Molecular Biology: The Use of Ultra-Small Gold Probes -- 8 Direct Peroxidase Labeling of Hybridization Probes and Chemiluminescence Detection -- 9 A Highly Sensitive Method for Detecting Peroxidase in In Situ Hybridization or Immunohistochemical Assays -- 10 The SNAP System -- II Specialized Nonradioactive Detection Systems -- 11 Overview of Colorimetric, Chemiluminometric, and Fluorimetric Detection Systems -- 12 Colorimetric Systems -- 13 Luminescence Systems -- 14 Fluorescence Systems -- III Enhanced Systems -- 15 Overview of Amplification Systems -- 16 Enhanced Signal Generation by Target Amplification -- 17 Signal Amplification System: Substrate Cascade -- IV Application Formats -- 18 Overview of Application Formats -- 19 Blot Formats: Nucleic Acids -- 20 Blot Formats: Proteins and Glycoproteins -- 21 In Situ Formats -- 22 Quantitative Formats -- Appendix I Suppliers of Reagents -- Appendix II Suppliers of Equipment.
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|a Loaded electronically.
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|a Electronic access restricted to members of the Holy Cross Community.
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|a Cell biology.
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|a Biochemistry.
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