|
|
|
|
LEADER |
00000nam a22000005i 4500 |
001 |
b3235557 |
003 |
MWH |
005 |
20191027181919.0 |
007 |
cr nn 008mamaa |
008 |
121227s1991 ne | s |||| 0|eng d |
020 |
|
|
|a 9789401133043
|
024 |
7 |
|
|a 10.1007/978-94-011-3304-3
|2 doi
|
035 |
|
|
|a (DE-He213)978-94-011-3304-3
|
050 |
|
4 |
|a E-Book
|
072 |
|
7 |
|a MJCL
|2 bicssc
|
072 |
|
7 |
|a MED062000
|2 bisacsh
|
072 |
|
7 |
|a MJCL
|2 thema
|
245 |
1 |
0 |
|a Human Cancer in Primary Culture, A Handbook
|h [electronic resource] /
|c edited by John Masters.
|
250 |
|
|
|a 1st ed. 1991.
|
264 |
|
1 |
|a Dordrecht :
|b Springer Netherlands :
|b Imprint: Springer,
|c 1991.
|
300 |
|
|
|a X, 299 p.
|b online resource.
|
336 |
|
|
|a text
|b txt
|2 rdacontent
|
337 |
|
|
|a computer
|b c
|2 rdamedia
|
338 |
|
|
|a online resource
|b cr
|2 rdacarrier
|
347 |
|
|
|a text file
|b PDF
|2 rda
|
490 |
1 |
|
|a Developments in Oncology ;
|v 64
|
490 |
1 |
|
|a Springer eBook Collection
|
505 |
0 |
|
|a One -- 1 Characterization -- 2 Serum-Free Media -- 3 Differentiation Potential of Cancer Cells -- 4 Spheroids and Xenografts -- 5 Predictive Assays for Drug and Radiation Resistance -- Two -- 6 Colorectum -- 7 Testicular Germ Cell Tumours -- 8 Epidermis -- 9 Lung Cancer -- 10 Brain -- 11 Ovarian Tumours -- 12 Prostate -- 13 Breast Cancer.
|
520 |
|
|
|a Growing human cancer cells in primary culture requires patience, intuition, care and experience. This is one of the few areas where the wrinkled senior scientist can be more productive than the bright young post-doc. There are few mechanical aids, no automated procedures, and kits are unheard-of. There is no right way to do it and every tumour is different. But this book will make it easier! Chapter 1 on characterization is essential reading. Much published work is useless because of the failure to take two simple steps to characterise the cells. The first step is to fully record all the clinical data - the absence of this information can render the work valueless. The second step is to confirm the origin of the tissue to exclude cross contamination. The wastage of years of work can be avoided with the use of a simple DNA preparation with a couple of commercially-available probes. Chapter 2 describes the development of serum-free media. This is a goal many would like to achieve, particularly if someone else does it, as it is laborious and empirical. Defined serum-free medium is essential for studies of growth factors and has major advantages in the commercial preparation of cell products and other applications.
|
590 |
|
|
|a Loaded electronically.
|
590 |
|
|
|a Electronic access restricted to members of the Holy Cross Community.
|
650 |
|
0 |
|a Cancer research.
|
650 |
|
0 |
|a Animal anatomy.
|
690 |
|
|
|a Electronic resources (E-books)
|
700 |
1 |
|
|a Masters, John.
|e editor.
|4 edt
|4 http://id.loc.gov/vocabulary/relators/edt
|
710 |
2 |
|
|a SpringerLink (Online service)
|
773 |
0 |
|
|t Springer eBooks
|
830 |
|
0 |
|a Developments in Oncology ;
|v 64
|
830 |
|
0 |
|a Springer eBook Collection.
|
856 |
4 |
0 |
|u https://holycross.idm.oclc.org/login?auth=cas&url=https://doi.org/10.1007/978-94-011-3304-3
|3 Click to view e-book
|t 0
|
907 |
|
|
|a .b32355579
|b 04-18-22
|c 02-26-20
|
998 |
|
|
|a he
|b 02-26-20
|c m
|d @
|e -
|f eng
|g ne
|h 0
|i 1
|
912 |
|
|
|a ZDB-2-SME
|
912 |
|
|
|a ZDB-2-BAE
|
950 |
|
|
|a Medicine (Springer-11650)
|
902 |
|
|
|a springer purchased ebooks
|
903 |
|
|
|a SEB-COLL
|
945 |
|
|
|f - -
|g 1
|h 0
|j - -
|k - -
|l he
|o -
|p $0.00
|q -
|r -
|s b
|t 38
|u 0
|v 0
|w 0
|x 0
|y .i21487224
|z 02-26-20
|
999 |
f |
f |
|i 9a8ab9e3-dacd-5490-b150-606833cd4b1c
|s bc4d18bc-3b34-5736-9862-f618e018c83c
|t 0
|
952 |
f |
f |
|p Online
|a College of the Holy Cross
|b Main Campus
|c E-Resources
|d Online
|t 0
|e E-Book
|h Library of Congress classification
|i Elec File
|