Genetic Library Construction and Screening Advanced Techniques and Applications / edited by R.C. Bird, Bruce F. Smith.

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Bibliographic Details
Corporate Author: SpringerLink (Online service)
Other Authors: Bird, R.C (Editor), Smith, Bruce F. (Editor)
Format: eBook
Language:English
Published: Berlin, Heidelberg : Springer Berlin Heidelberg : Imprint: Springer, 2002.
Edition:1st ed. 2002.
Series:Springer Lab Manuals
Springer eBook Collection.
Subjects:
Online Access:Click to view e-book
Holy Cross Note:Loaded electronically.
Electronic access restricted to members of the Holy Cross Community.
Table of Contents:
  • PCR and cDNA Cloning Techniques
  • 1 Strategies for cDNA Cling and Mapping RNA Transcripts
  • 2 Cloning PCR Products
  • 3 Site Directed Deletion, Insertion, and Substitution Using PCR
  • 4 Long RT-PCR Cloning — Amplification of Full-Length Enterovirus Genomes
  • 5 Construction of cDNA Libraries from Small Quantities of Total RNA Using Template Switching Catalyzed by M-MLV Reverse Transcriptase
  • Subtractive and Differential Display Techniques
  • 6 Subtractive Hybridization and cDNA Cloning
  • 7 Differential Display to Identify Steroid-Induced Genes in Endocrine Cells
  • Specialized Cloning and Library Screening Strategies
  • 8 Two Hyrid cDNA Cloning
  • 9 Yeast One and Two Hyrid cDNA Cloning
  • 10 High-Throughput Library Screening by Fluorescent Hybridisations on Gridded Membranes
  • 11 Identification of Cell Targeting Ligands Using Random Peptide-Presenting Phage Libraries.