Doubled Haploid Production in Crop Plants A Manual

Doubled Haploid Production in Crop Plants A Manual / edited by M. Maluszynski, Kenneth Kasha, B.P. Forster, I. Szarejko.

The production of doubled haploids has become a necessary tool in advanced plant breeding institutes and commercial companies for breeding many crop species. However, the development of new, more efficient and cheaper large scale production protocols has meant that doubled haploids are also recently...

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Bibliographic Details
Corporate Author: SpringerLink (Online service)
Other Authors: Maluszynski, M. (Editor), Kasha, Kenneth (Editor), Forster, B.P (Editor), Szarejko, I. (Editor)
Format: eBook
Language:English
Published: Dordrecht : Springer Netherlands : Imprint: Springer, 2003.
Edition:1st ed. 2003.
Series:Springer eBook Collection.
Subjects:
Plant science.
Botany.
Agriculture.
Trees.
Life sciences.
Electronic resources (E-books)
Online Access:Click to view e-book
Holy Cross Note:Loaded electronically.
Electronic access restricted to members of the Holy Cross Community.
Table of Contents:
  • 1. Production of doubled haploids in crop plants. An introduction
  • 2.1. Doubled haploid production in barley using the Hordeum bulbosum (L.) technique
  • 2.2. The Hordeum bulbosum (L.) method
  • 2.3 Anther culture in barley
  • 2.4. Barley anther culture
  • 2.5. Anther culture for doubled haploid production in barley (Hordeum vulgare L.)
  • 2.6. Barley isolated microspore culture protocol
  • 2.7. Barley isolated microspore culture (IMC) method
  • 2.8. Doubled haploid production in wheat through wide hybridization
  • 2.9. Protocol of wheat (Triticum eastivum L.) anther culture
  • 2.10. Protocol for producing doubled haploid plants from anther culture of wheat (Triticum aestivum L.)
  • 2.11. Wheat anther culture
  • 2.12. Haploid wheat isolated microspore culture protocol
  • 2.13. Production of doubled haploids in wheat (Triticum aestivum L.) through microspore embryogenesis triggered by inducer chemicals
  • 2.14. Isolated microspore culture in maize (Zea mays L.), production of doubled-haploid via induced androgenesis
  • 2.15. Anther culture of maize (Zea mays L.)
  • 2.16. Laboratory protocol for anther culture technique in rice
  • 2.17. Triticale anther culture
  • 2.18. Protocol for anther culture in hexaploid triticale (x Triticosecale Wittm.)
  • 2.19. Protocol of triticale (x Triticosecale Wittmack) microspore culture
  • 2.20. Protocol for doubled haploid production in hexaploid triticale (x Triticosecale Wittm.) by crosses with maize
  • 2.21. Protocol for rye anther culture
  • 2.22. Microspore culture of rye
  • 2.23. Oat haploids from wide hybridization
  • 2.24. Haploid and doubled haploid production in dumm wheat by wide hybridization
  • 2.25. Haploid and doubled haploid production in dumm wheat by anther culture
  • 2.26. Anther culture and isolated microspore culture in timothy
  • 2.27. Doubled haploid induction in ryegrass and other grasses
  • 2.28. Microspore culture in rapeseed (Brassica napus L.)
  • 2.29. Protocol for broccoli microspore culture
  • 2.30. Microspore culture of Brassica species
  • 2.31. Protocol for microspore culture in Brassica
  • 2.32. Anther and microspore culture in tobacco
  • 2.33. Haploid production of potatoes by anther culture
  • 2.34. Anther culture through direct embryogenesis in a genetically diverse range of potato (Solanum) species and their interspecific and intergeneric hybrids
  • 2.35. Potato haploid technologies
  • 2.36. Anther culture of linseed (Linum usitatissimum L.)
  • 2.37. Doubled haploid production of sugar beet (Beta vulgaris L.)
  • 2.38. Asparagus microspore and anther culture
  • 2.39. In vitro gynogenesis induction and doubled haploid production in onion (Allium cepa L.)
  • 2.40. Haploid induction in onion via gynogenesis
  • 2.41. In vitro androgenesis in apple
  • 2.42. Doubled haploid production in poplar
  • 2.43. Oak anther culture
  • 2.44. Haploids and doubled haploids in Citrus spp.
  • 3. Published protocols for other crop plant species
  • 4.1. Doubled haploids in breeding
  • 4.2. Doubled haploid mutant production
  • 4.3. Barley microspore transformation protocol by biolistic gun
  • 4.4. Doubled haploids in genetic mapping and genomics
  • 5.1. Cytogenetic tests for ploidy level analyses — chromosome counting
  • 5.2. Ploidy determination using flow cytometry
  • 6.1. Major media composition
  • 6.2. Basic equipment for maize microspore culture laboratory
  • List of contributors.

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