Differential-Display Reverse Transcription-PCR (DDRT-PCR) by Sergio Colonna-Romano, Antonella Leone, Bruno Maresca.
Identification of differentially expressed genes is one of the major challenges in molecular biology. Several techniques allow the cloning of such sequences. However, methods such as RNA subtraction or differential hybridization are time-consuming and require large amounts of mRNA. Recently, a new a...
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