Using Mass Spectrometry for Biochemical Studies on Enzymatic Domains from Polyketide Synthases by Matthew Jenner.

This thesis reports studies on the substrate specificity of crucial ketosynthase (KS) domains from trans-AT Polyketide Synthases (PKSs). Using a combination of electrospray ionisation-mass spectrometry (ESI-MS) and simple N-acetyl cysteamine (SNAC) substrate mimics, Matthew Jenner has successfully s...

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Bibliographic Details
Main Author: Jenner, Matthew (Author)
Corporate Author: SpringerLink (Online service)
Format: eBook
Language:English
Published: Cham : Springer International Publishing : Imprint: Springer, 2016.
Edition:1st ed. 2016.
Series:Springer Theses, Recognizing Outstanding Ph.D. Research,
Springer eBook Collection.
Subjects:
Online Access:Click to view e-book
Holy Cross Note:Loaded electronically.
Electronic access restricted to members of the Holy Cross Community.
Table of Contents:
  • Introduction
  • Materials and Methods
  • Substrate Specificity of Ketosynthase Domains Part I: β-Branched Acyl Chains
  • Substrate Specificity of Ketosynthase Domains Part II: Amino Acid-Containing Acyl Chains
  • Synthesis of Acyl-Acyl Carrier Proteins and their use in Studying Polyketide Synthase Enzymology
  • Substrate Specificity of Ketosynthase Domains Part III: Elongation-Based Substrate Specificity.