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| 049 |
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|a HCDD
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| 245 |
0 |
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|a Mutation detection :
|b a practical approach /
|c edited by R.G.H. Cotton, E. Edkins, and S. Forrest.
|
| 260 |
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|a Oxford ;
|a New York :
|b IRL Press at Oxford University Press,
|c ©1998.
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| 300 |
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|a 1 online resource (xx, 242 pages) :
|b illustrations
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 347 |
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|a data file
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1 |
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|a The practical approach series ;
|v 188
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| 504 |
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|a Includes bibliographical references and index.
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| 506 |
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|3 Use copy
|f Restrictions unspecified
|2 star
|5 MiAaHDL
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| 520 |
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|a Mutation detection is increasingly undertaken in a wide spectrum of research areas: in medicine it is fundamental in isolating disease genes and diagnosis, and is especially important in cancer research; in biology, commercially important genes can be identified by the mutations they contain. But mutation detection is time-consuming and expensive. This volume offers tried and tested protocols for a range of detection methods, from the labs of researchers in the field.
|
| 533 |
|
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|a Electronic reproduction.
|b [Place of publication not identified] :
|c HathiTrust Digital Library,
|d 2010.
|5 MiAaHDL
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| 538 |
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|a Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002.
|u http://purl.oclc.org/DLF/benchrepro0212
|5 MiAaHDL
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| 583 |
1 |
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|a digitized
|c 2010
|h HathiTrust Digital Library
|l committed to preserve
|2 pda
|5 MiAaHDL
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| 588 |
0 |
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|a Print version record.
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| 505 |
0 |
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|a Cover -- Contents -- List of Contributors -- Abbreviations -- Introduction -- References -- 1. Single-strand conformation polymorphism analysis -- 1. Introduction -- 2. PCR-SSCP using polyacrylamide slab gel -- PCR Optimization and primer design -- Pre-amplification and isolation by agarose gel electrophoresis -- PCR using [[Sup(32)]P]deoxynucleotide triphosphate -- Removal of 3' appendage -- SSCP gel electrophoresis -- Interpretation of autoradiogram -- Re-amplification and direct sequencing -- Gel matrices other than polyacrylamide.
|
| 505 |
8 |
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|a Restriction endonuclease fingerprinting and dideoxy fingerprinting -- 3. Fluorescent SSCP in an automated DNA sequencer -- Primer design in post-PCR fluorescent labelling -- Fluorescent labelling by 3' exchange reaction -- SSCP in capillary electrophoresis (CE-SSCP) -- Data processing -- Acknowledgements -- References -- 2. Single-stranded conformation polymorphism and heteroduplex analysis -- 1. Introduction -- 2. Optimization of the PCR reaction -- 3. SSCP sample prepration -- 4. Optimization of SSCP/HA detection -- 5. Multiplexing -- 6. Interpretation of results -- 7. Applications.
|
| 505 |
8 |
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|a 8. Other methods -- References -- 3. Comprehensive mutation detection with denaturing gradient gel electrophoresis -- 1. Introduction -- The scope of DGGE, its distinctive capabilities, and the nature of results -- 2. Background -- 3. Basic principle, the physical properties of DNA -- 4. Overview of the procedures in searching for mutants -- Defining segments for scrutiny -- Sample preparation -- Gradient and velocity separations -- Features of the gel patterns -- Discrimination of zygozygosity -- Comments -- 5. Use of the psoralen cross-link as a clamp -- The psoralen protocol.
|
| 505 |
8 |
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|a 6. Computational tools -- What is a meltmap? -- Meltmap protocol -- Predicting electrophoretic separations -- Computer operations for MUTRAV -- 7. Other members of the DGGE family -- Gel separations in a uniform, partially denaturing environment -- Capillary electrophoresis -- The thermal gradient -- The temperature ramp -- 2D length and gradient separations -- 8. End notes -- Acknowledgments -- References -- 4. Cleavage using RNase to detect mutations -- 1. Introduction -- 2. RNase protection assay for mutation detection -- Evaluation of the sensitivity -- Source material.
|
| 505 |
8 |
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|a PCR for RNase protection assay -- RNA probe preparation -- RNase protection -- Detection of digested probe -- Mutation detection by sequencing of the PCR products -- Other modified methodologies for mutation detection -- Acknowledgements -- References -- 5. Cleavage of mismatched bases using chemical reagents -- 1. Introduction -- 2. Basic procedures -- Comments on the basic procedures -- 3. Ultra fast chemical mismatch detection -- Labelling -- Solid phase -- Comments -- References -- 6. Mutation detection using T4 endonuclease VII -- 1. Introduction -- 2. The biology of Endo VII.
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| 650 |
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0 |
|a Mutation (Biology)
|v Laboratory manuals.
|
| 650 |
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0 |
|a Molecular genetics
|v Laboratory manuals.
|
| 650 |
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0 |
|a Chromosome abnormalities
|v Laboratory manuals.
|
| 650 |
|
0 |
|a Mutation (Biology)
|
| 650 |
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|a Chromosome abnormalities.
|
| 650 |
|
7 |
|a SCIENCE
|x Life Sciences
|x Genetics & Genomics.
|2 bisacsh
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| 650 |
|
7 |
|a Chromosome abnormalities
|2 fast
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| 650 |
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7 |
|a Molecular genetics
|2 fast
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| 650 |
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7 |
|a Mutation (Biology)
|2 fast
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| 650 |
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7 |
|a Genmutation
|2 gnd
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| 650 |
|
7 |
|a Nachweis
|2 gnd
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| 655 |
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|a Laboratory manuals
|2 fast
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| 655 |
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7 |
|a Laboratory manuals.
|2 lcgft
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| 655 |
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|a Manuels de laboratoire.
|2 rvmgf
|
| 700 |
1 |
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|a Cotton, Richard G. H.
|
| 700 |
1 |
|
|a Edkins, E.
|q (Edward)
|1 https://id.oclc.org/worldcat/entity/E39PCjJY4XK4jXTGxcxBpjKmkC
|
| 700 |
1 |
|
|a Forrest, S.
|q (Sue)
|1 https://id.oclc.org/worldcat/entity/E39PCjF8gdPgfpxBYg83BD9Wwy
|
| 758 |
|
|
|i has work:
|a Mutation detection (Text)
|1 https://id.oclc.org/worldcat/entity/E39PCGw8XgDVW3jVggthGXv6Td
|4 https://id.oclc.org/worldcat/ontology/hasWork
|
| 776 |
0 |
8 |
|i Print version:
|t Mutation detection.
|d Oxford ; New York : IRL Press at Oxford University Press, ©1998
|z 0199636575
|z 0199636567
|w (DLC) 97051537
|w (OCoLC)38249893
|
| 830 |
|
0 |
|a Practical approach series ;
|v 188.
|
| 856 |
4 |
0 |
|u https://ebookcentral.proquest.com/lib/holycrosscollege-ebooks/detail.action?docID=1044783
|y Click for online access
|
| 903 |
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|a EBC-AC
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| 994 |
|
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|a 92
|b HCD
|