|
|
|
|
LEADER |
00000cam a2200000Ma 4500 |
001 |
ocn314216993 |
003 |
OCoLC |
005 |
20240809213013.0 |
006 |
m o d |
007 |
cr zn||||||||| |
008 |
040402s2004 enka ob 001 0 eng d |
010 |
|
|
|a 2004299783
|
040 |
|
|
|a CN8ML
|b eng
|e pn
|c CN8ML
|d OCLCQ
|d N$T
|d E7B
|d OCLCQ
|d IDEBK
|d MERUC
|d OCLCQ
|d GZM
|d OCLCO
|d OCLCF
|d OCLCQ
|d NLGGC
|d YDXCP
|d EBLCP
|d MHW
|d DEBSZ
|d OCLCQ
|d TOA
|d AGLDB
|d OTZ
|d OCLCO
|d OCLCQ
|d OCLCA
|d OCLCQ
|d OCLCO
|d OCLCQ
|d STF
|d OCLCO
|d VNS
|d OCLCO
|d VTS
|d OCLCO
|d CEF
|d COCUF
|d AU@
|d OCLCO
|d LHU
|d FVL
|d OCLCQ
|d OCLCA
|d S9I
|d YOU
|d CANPU
|d REC
|d CNTRU
|d MT4IT
|d LOA
|d K6U
|d VT2
|d OCLCO
|d OCLCQ
|d SFB
|d OCLCA
|d AJS
|d OCLCO
|d OCLCQ
|d YDX
|d OCLCO
|d OCLCL
|d SXB
|
019 |
|
|
|a 316043706
|a 455959813
|a 476259877
|a 646784754
|a 756879021
|a 994691172
|a 1035659532
|a 1044148989
|a 1044294206
|a 1056326933
|a 1056353918
|a 1059562508
|a 1059786311
|a 1077822793
|a 1097260816
|a 1099557162
|a 1107715826
|a 1109953112
|a 1111283265
|a 1117840156
|a 1125449263
|a 1132017190
|a 1136452650
|
020 |
|
|
|a 9780191516450
|q (electronic bk.)
|
020 |
|
|
|a 0191516457
|q (electronic bk.)
|
020 |
|
|
|a 9786610845293
|
020 |
|
|
|a 6610845298
|
020 |
|
|
|a 9780199638734
|
020 |
|
|
|a 019963873X
|
020 |
|
|
|z 019963873X
|q (Paper)
|
020 |
|
|
|z 0199638748
|q (Cloth)
|
020 |
|
|
|z 9780199638741
|
027 |
|
|
|a MYILIB_CUp
|
035 |
|
|
|a (OCoLC)314216993
|z (OCoLC)316043706
|z (OCoLC)455959813
|z (OCoLC)476259877
|z (OCoLC)646784754
|z (OCoLC)756879021
|z (OCoLC)994691172
|z (OCoLC)1035659532
|z (OCoLC)1044148989
|z (OCoLC)1044294206
|z (OCoLC)1056326933
|z (OCoLC)1056353918
|z (OCoLC)1059562508
|z (OCoLC)1059786311
|z (OCoLC)1077822793
|z (OCoLC)1097260816
|z (OCoLC)1099557162
|z (OCoLC)1107715826
|z (OCoLC)1109953112
|z (OCoLC)1111283265
|z (OCoLC)1117840156
|z (OCoLC)1125449263
|z (OCoLC)1132017190
|z (OCoLC)1136452650
|
037 |
|
|
|a 084529
|b MIL
|
050 |
|
4 |
|a QR342
|b .P43 2004eb
|
055 |
1 |
3 |
|a QR342
|b .P43 2004eb
|
072 |
|
7 |
|a SCI
|x 008000
|2 bisacsh
|
072 |
|
7 |
|a SCI
|x 045000
|2 bisacsh
|
049 |
|
|
|a HCDD
|
245 |
0 |
0 |
|a Phage display :
|b a practical approach /
|c edited by Tim Clackson, Henry B. Lowman.
|
260 |
|
|
|a Oxford ;
|a New York :
|b Oxford University Press,
|c ©2004.
|
300 |
|
|
|a 1 online resource (xxiv, 332 pages) :
|b illustrations
|
336 |
|
|
|a text
|b txt
|2 rdacontent
|
337 |
|
|
|a computer
|b c
|2 rdamedia
|
338 |
|
|
|a online resource
|b cr
|2 rdacarrier
|
347 |
|
|
|a data file
|2 rda
|
490 |
1 |
|
|a Practical approach series ;
|v no. 266
|
504 |
|
|
|a Includes bibliographical references and index.
|
588 |
0 |
|
|a Print version record.
|
505 |
0 |
|
|a Cover -- Contents -- Protocol list -- List of abbreviations -- List of contributors -- 1 Introduction to phage biology and phage display -- 1 Introduction -- 2 Biology of filamentous phage -- 2.1 Introduction -- 2.2 Structure of the phage particle -- 2.3 Infection -- 2.4 Replication -- 2.5 Genes and gene expression -- 2.6 Physiology of phage assembly -- 2.7 The mechanics of phage assembly -- 3 Coat proteins used for display -- 3.1 pVIII -- 3.2 pIll -- 4 Starting a phage display project -- 4.1 Feasibility of display -- 4.2 Phage or phagemid vector? -- 4.3 Polyvalent or monovalent display? -- 4.4 Helper phage -- 4.5 General protocols for phage preparation and quantitation -- 5 General principles of a phage display project -- 5.1 Making a library -- 5.2 Selection -- 5.3 Analysis of clones -- 6 Common problems -- 6.1 Library quality -- 6.2 Expression editing -- 6.3 Over-selection -- 7 Alternative display systems -- 8 Commercial sources of phage display libraries and kits -- References -- 2 Constructing phage display libraries by oligonucleotide-directed mutagenesis -- 1 Introduction -- 2 Considerations for library design -- 2.1 Site-directed mutagenesis -- 2.2 Degenerate codon design -- 2.3 Theoretical versus actual diversity -- 3 Oligonucleotide-directed mutagenesis -- 3.1 Oligonucleotide-directed mutagenesis versus cassette mutagenesis -- 3.2 The chemistry and biology of oligonucleotide-directed mutagenesis -- 3.3 Construction of an inactive template -- 4 Library construction and storage -- 4.1 Preparation of single-stranded DNA template -- 4.2 In vitro synthesis of heteroduplex CCC-dsDNA -- 4.3 E.coli electroporation and production of library phage -- 4.4 Library storage and reinfection -- 5 Biological reagents -- References -- 3 In vitro DNA recombination -- 1 Introduction -- 2 Background to in vitro DNA recombination -- 2.1 Use of in vitro DNA recombination in directed evolution -- 2.2 Applications of in vitro DNA recombination -- 2.3 Recombination statistics -- 3 Methods for in vitro DNA recombination -- 3.1 Stemmer method -- 3.2 Random DNA fragmentation with endonuclease V from E. coli -- 3.3 Random priming recombination -- 3.4 Staggered Extension Process (StEP) -- 3.5 In vitro heteroduplex formation and in vivo repair (heteroduplex recombination) -- 3.6 Choice of recombination method -- 3.7 Removal of background -- 3.8 Technical tips -- References -- 4 Phage selection strategies for improved affinity and specificity of proteins and peptides -- 1 Introduction -- 2 Vector considerations -- 2.1 Monovalent and polyvalent phage display -- 2.2 Confirming display -- 2.3 Protein expression from phagemid vectors -- 2.4 Vector construction and phagemid preparation -- 3 Library design -- 3.1 Hard randomization -- 3.2 Soft randomization -- 4 Target presentation -- 4.1 Direct immobilization -- 4.2 Solution binding -- 4.3 Blocking -- 4.4 Pilot selection -- 5 Selection -- 5.1 Binding buffer considerations -- 5.2 Stringency of selection -- 5.3 Competitive selection -- 5.4 Elution of bound phage -- 5.5 Amplification -- 5.6 Monitori.
|
520 |
|
|
|a This new book aims to enable researchers to design and undertake all aspects of a phage display project, from designing an experimental strategy and constructing a library to performing selections and analyzing the results. All of the protocols and chapters are extensively cross-referenced, allowing readers to move beyond the specific examples provided in order to customize the procedures for their own protein or selection system of interest. Phage Display is an up-to-date, . comprehensive and integrated experimental guide to the technique, which is essential reading for anyone currently using.
|
650 |
|
0 |
|a Bacteriophages.
|
650 |
|
0 |
|a Viral proteins.
|
650 |
|
0 |
|a Microbial biotechnology.
|
650 |
|
7 |
|a SCIENCE
|x Life Sciences
|x Biology.
|2 bisacsh
|
650 |
|
7 |
|a SCIENCE
|x Life Sciences
|x Microbiology.
|2 bisacsh
|
650 |
|
7 |
|a Bacteriophages
|2 fast
|
650 |
|
7 |
|a Microbial biotechnology
|2 fast
|
650 |
|
7 |
|a Viral proteins
|2 fast
|
700 |
1 |
|
|a Clackson, Tim.
|
700 |
1 |
|
|a Lowman, Henry B.
|
758 |
|
|
|i has work:
|a Phage display (Text)
|1 https://id.oclc.org/worldcat/entity/E39PCG6xpDXTJW6DDdQjHdHV4q
|4 https://id.oclc.org/worldcat/ontology/hasWork
|
776 |
0 |
8 |
|i Print version:
|t Phage display.
|d Oxford ; New York : Oxford University Press, ©2004
|z 0199638748
|w (DLC) 2004299783
|w (OCoLC)54904081
|
830 |
|
0 |
|a Practical approach series ;
|v 266.
|
856 |
4 |
0 |
|u https://ebookcentral.proquest.com/lib/holycrosscollege-ebooks/detail.action?docID=422829
|y Click for online access
|
903 |
|
|
|a EBC-AC
|
994 |
|
|
|a 92
|b HCD
|