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OCoLC |
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20240809213013.0 |
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100524s2010 xx o 000 0 eng d |
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|b eng
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|d OCLCQ
|d MHW
|d OCLCQ
|d DEBSZ
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|d MERUC
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| 066 |
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|c (S
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|a 9781444318616
|q (electronic bk.)
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|a 1444318616
|q (electronic bk.)
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|a (OCoLC)630541245
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|a QH442.2 .B76 2010
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| 049 |
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|a HCDD
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| 100 |
1 |
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|a Brown, T. A.
|q (Terence A.)
|1 https://id.oclc.org/worldcat/entity/E39PBJhX394kWkjcJgbBhfhWDq
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| 245 |
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|a Gene Cloning and DNA Analysis :
|b an Introduction.
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| 250 |
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|a 6th ed.
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| 260 |
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|a Chichester :
|b John Wiley & Sons,
|c 2010.
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| 300 |
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|a 1 online resource (338 pages)
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| 336 |
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|a text
|b txt
|2 rdacontent
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|a computer
|b c
|2 rdamedia
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|a online resource
|b cr
|2 rdacarrier
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| 505 |
0 |
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|6 880-01
|a GENE CLONING AND DNA ANALYSIS; Contents; TO THE SIXTH EDITION Preface to the Sixth Edition; PART I The Basic Principles of Gene Cloning and DNA Analysis; Chapter 1 Why Gene Cloning and DNA Analysis are Important; Chapter 2 Vectors for Gene Cloning:Plasmids and Bacteriophages; Chapter 3 Purification of DNA from Living Cells; Chapter 4 Manipulation of Purified DNA; Chapter 5 Introduction of DNA into Living Cells; Chapter 6 Cloning Vectors for E.coli; Chapter 7 Cloning Vectors for Eukaryotes; Chapter 8 How to Obtain a Clone of a Specific Gene; Chapter 9 The Polymerase Chain Reaction.
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|a PART II The Applications of Gene Cloning and DNA Analysis in ResearchChapter 10 Sequencing Genes and Genomes; Chapter 11 Studying Gene Expression and Function; Chapter 12 Studying Genomes; PART III The Applications of Gene Cloning and DNA Analysis in Biotechnology; Chapter 13 Production of Protein from Cloned Genes; Chapter 14 Gene Cloning and DNA Analysis in Medicine; Chapter 15 Gene Cloning and DNA Analysis in Agriculture; C.
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| 520 |
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|a Known world-wide as the standard introductory text to this important and exciting area, the sixth edition of Gene Cloning and DNA Analysis addresses new and growing areas of research whilst retaining the philosophy of the previous editions. Assuming the reader has little prior knowledge of the subject, its importance, the principles of the techniques used and their applications are all carefully laid out, with over 250 clearly presented four-colour illustrations. In addition to a number of informative changes to the text throughout the book, the final four chapters have been significantly upda.
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|a Print version record.
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| 650 |
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|a DNA.
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| 650 |
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|a Molecular cloning.
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| 650 |
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|a Nucleotide sequence.
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| 650 |
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7 |
|a DNA
|2 fast
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| 650 |
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7 |
|a Molecular cloning
|2 fast
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| 650 |
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7 |
|a Nucleotide sequence
|2 fast
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| 758 |
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|i has work:
|a Gene cloning and DNA analysis (Text)
|1 https://id.oclc.org/worldcat/entity/E39PCGBV799CPHjKpdgFG38tDm
|4 https://id.oclc.org/worldcat/ontology/hasWork
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| 776 |
1 |
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|z 9781405181730
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| 856 |
4 |
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|u https://ebookcentral.proquest.com/lib/holycrosscollege-ebooks/detail.action?docID=530049
|y Click for online access
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| 880 |
0 |
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|6 505-00/(S
|a GENE CLONING AND DNA ANALYSIS -- Contents -- TO THE SIXTH EDITION Preface to the Sixth Edition -- PART I The Basic Principles of Gene Cloning and DNA Analysis -- Chapter 1 Why Gene Cloning and DNA Analysis are Important -- 1.1 The early development of genetics -- 1.2 The advent of gene cloning and the polymerase chain reaction -- 1.3 What is gene cloning-- 1.4 What is PCR-- 1.5 Why gene cloning and PCR are so important -- 1.5.1 Obtaining a pure sample of a gene by cloning -- 1.5.2 PCR can also be used to purify a gene -- 1.6 How to find your way through this book -- Chapter 2 Vectors for Gene Cloning:Plasmids and Bacteriophages -- 2.1 Plasmids -- 2.1.1 Size and copy number -- 2.1.2 Conjugation and compatibility -- 2.1.3 Plasmid classification -- 2.1.4 Plasmids in organisms other than bacteria -- 2.2 Bacteriophages -- 2.2.1 The phage infection cycle -- 2.2.2 Lysogenic phages -- Gene organization in the λ DNA molecule -- The linear and circular forms of λ DNA -- M13 -- a filamentous phage -- 2.2.3 Viruses as cloning vectors for other organisms -- Chapter 3 Purification of DNA from Living Cells -- 3.1 Preparation of total cell DNA -- 3.1.1 Growing and harvesting a bacterial culture -- 3.1.2 Preparation of a cell extract -- 3.1.3 Purification of DNA from a cell extract -- Removing contaminants by organic extraction and enzyme digestion -- Using ion-exchange chromatography to purify DNA from a cell extract -- 3.1.4 Concentration of DNA samples -- 3.1.5 Measurement of DNA concentration -- 3.1.6 Other methods for the preparation of total cell DNA -- 3.2 Preparation of plasmid DNA -- 3.2.1 Separation on the basis of size -- 3.2.2 Separation on the basis of conformation -- Alkaline denaturation -- Ethidium bromide-caesium chloride density gradient centrifugation -- 3.2.3 Plasmid amplification -- 3.3 Preparation of bacteriophage DNA.
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|6 505-00/(S
|a 3.3.1 Growth of cultures to obtain a high λ titer -- 3.3.2 Preparation of non-lysogenic λ phages -- 3.3.3 Collection of phages from an infected culture -- 3.3.4 Purification of DNA from λ phage particles -- 3.3.5 Purification of M13 DNA causes few problems -- Chapter 4 Manipulation of Purified DNA -- 4.1 The range of DNA manipulative enzymes -- 4.1.1 Nucleases -- 4.1.2 Ligases -- 4.1.3 Polymerases -- 4.1.4 DNA modifying enzymes -- 4.2 Enzymes for cutting DNA -- restriction endonucleases -- 4.2.1 The discovery and function of restriction endonucleases -- 4.2.2 Type II restriction endonucleases cut DNA at specific nucleotide sequences -- 4.2.3 Blunt ends and sticky ends -- 4.2.4 The frequency of recognition sequences in a DNA molecule -- 4.2.5 Performing a restriction digest in the laboratory -- 4.2.6 Analyzing the result of restriction endonuclease cleavage -- Separation of molecules by gel electrophoresis -- Visualizing DNA molecules in an agarose gel -- 4.2.7 Estimation of the sizes of DNA molecules -- 4.2.8 Mapping the positions of different restriction sites in a DNA molecule -- 4.2.9 Special gel electrophoresis methods for separating larger molecules -- 4.3 Ligation -- joining DNA molecules together -- 4.3.1 The mode of action of DNA ligase -- 4.3.2 Sticky ends increase the efficiency of ligation -- 4.3.3 Putting sticky ends onto a blunt-ended molecule -- Linkers -- Adaptors -- Producing sticky ends by homopolymer tailing -- 4.3.4 Blunt end ligation with a DNA topoisomerase -- Chapter 5 Introduction of DNA into Living Cells -- 5.1 Transformation -- the uptake of DNA by bacterial cells -- 5.1.1 Not all species of bacteria are equally efficient at DNA uptake -- 5.1.2 Preparation of competent E.coli cells -- 5.1.3 Selection for transformed cells -- 5.2 Identification of recombinants.
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|6 505-01/(S
|a 5.2.1 Recombinant selection with pBR322 -- insertional inactivation of an antibiotic resistance gene -- 5.2.2 Insertional inactivation does not always involve antibiotic resistance -- 5.3 Introduction of phage DNA into bacterial cells -- 5.3.1 Transfection -- 5.3.2 In vitro packaging of λ cloning vectors -- 5.3.3 Phage infection is visualized as plaques on an agar medium -- 5.4 Identification of recombinant phages -- 5.4.1 Insertional inactivation of a lacZ' gene carried by the phage vector -- 5.4.2 Insertional inactivation of the λ cI gene -- 5.4.3 Selection using the Spi phenotype -- 5.4.4 Selection on the basis of λ genome size -- 5.5 Introduction of DNA into non-bacterial cells -- 5.5.1 Transformation of individual cells -- 5.5.2 Transformation of whole organisms -- Chapter 6 Cloning Vectors for E.coli -- 6.1 Cloning vectors based on E.coli plasmids -- 6.1.1 The nomenclature of plasmid cloning vectors -- 6.1.2 The useful properties of pBR322 -- 6.1.3 The pedigree of pBR322 -- 6.1.4 More sophisticated E.coli plasmid cloning vectors -- pUC8 -- a Lac selection plasmid -- pGEM3Z -- in vitro transcription of cloned DNA -- 6.2 Cloning vectors based on M13 bacteriophage -- 6.2.1 How to construct a phage cloning vector -- 6.2.2 Hybrid plasmid-M13 vectors -- 6.3 Cloning vectors based on λ bacteriophage -- 6.3.1 Segments of the λ genome can impairing viability -- 6.3.2 Natural selection can be used to isolate modified λ that lack certain restriction sites -- 6.3.3 Insertion and replacement vectors -- Insertion vectors -- Replacement vectors -- 6.3.4 Cloning experiments with λ insertion or replacement vectors -- 6.3.5 Long DNA fragments can be cloned using a cosmid -- 6.4 8 and other high-capacity vectors enable genomic libraries to be constructed -- 6.5 Vectors for other bacteria -- Chapter 7 Cloning Vectors for Eukaryotes.
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|a 92
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