Methods in Bioengineering.

This unique volume presents leading-edge microfluidics methods used to handle, manipulate, and analyze cells, particles, and biological components (e.g., proteins and DNA) for microdiagnostics. The authors offer clear and detailed guidance on microfabrication techniques utilized to create microfluid...

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Bibliographic Details
Main Author: Zahn, Jeffrey D.
Format: eBook
Language:English
Published: Norwood : Artech House, 2009.
Series:Methods in bioengineering.
Subjects:
Online Access:Click for online access

MARC

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490 1 |a Methods in Bioengineering 
505 0 |a Methods in Bioengineering: Biomicrofabrication and Biomicrofluidics; Contents; Preface; Chapter 1 Microfabrication Techniques for Microfluidic Devices; 1.1 Introduction to microsystems and microfluidic devices; 1.2 Microfluidic systems: fabrication techniques; 1.3 Transfer processes; 1.3.1 Photolithography; 1.3.2 Molding; 1.4 Additive processes; 1.4.1 Growth of SiO2; 1.4.2 Deposition techniques; 1.5 Subtractive techniques; 1.5.1 Etching; 1.5.2 Chemical-mechanical polishing and planarization; 1.6 Bonding processes; 1.6.1 Lamination; 1.6.2 Wafer bonding methods; 1.7 Sacrificial layer techniques. 
505 8 |a 1.8 Packaging processes1.8.1 Dicing; 1.8.2 Electrical interconnection and wire bonding; 1.8.3 Fluidic interconnection in microfluidic systems; 1.9 Materials for microfluidic and bio-MEMS applications; 1.9.1 Glass, pyrex, and quartz; 1.9.2 Silicon; 1.9.3 Elastomers; 1.9.4 Polydimethylsiloxane; 1.9.5 Epoxy; 1.9.6 SU-8 thick resists; 1.9.7 Thick positive resists; 1.9.8 Benzocyclobutene; 1.9.9 Polyimides; 1.9.10 Polycarbonate; 1.9.11 Polytetrafluoroethylene; 1.10 Troubleshooting table; 1.11 Summary; References; Chapter 2 Micropumping and Microvalving; 2.1 Introduction. 
505 8 |a 2.2 Actuators for micropumps and microvalves2.2.1 Pneumatic actuators; 2.2.2 Thermopneumatic actuators; 2.2.3 Solid-expansion actuators; 2.2.4 Bimetallic actuators; 2.2.5 Shape-memory alloy actuators; 2.2.6 Piezoelectric actuators; 2.2.7 Electrostatic actuators; 2.2.8 Electromagnetic actuators; 2.2.9 Electrochemical actuators; 2.2.10 Chemical actuators; 2.2.11 Capillary-force actuators; 2.3 Micropumps; 2.3.1 Mechanical pump; 2.3.2 Nonmechanical pump; 2.4 Microvalves; 2.4.1 Mechanical valve; 2.4.2 Nonmechanical valve; 2.5 Outlook; 2.6 Troubleshooting; 2.7 Summary points; References. 
505 8 |a Chapter 3 Micromixing Within Microfluidic Devices3.1 Introduction; 3.2 Materials; 3.2.1 Microfluidic mixing devices; 3.2.2 Microfluidic interconnects; 3.2.3 Optical assembly; 3.2.4 Required reagents; 3.3 Experimental design and methods; 3.3.1 Passive micromixers; 3.3.2 Active micromixers; 3.3.3 Multiphase mixers; 3.4 Data acquisition, anticipated results, and interpretation; 3.4.1 Computer acquisition; 3.4.2 Performance metrics, extent of mixing, reaction monitoring; 3.5 Discussion and commentary; 3.6 Troubleshooting; 3.7 Application notes; 3.8 Summary points; References. 
505 8 |a Chapter 4 On-Chip Electrophoresis and Isoelectric Focusing Methods for Quantitative Biology4.1 Introduction; 4.1.1 Microfluidic electrophoresis supports quantitative biology and medicine; 4.1.2 Biomedical applications of on-chip electrophoresis; 4.2 Materials; 4.2.1 Reagents; 4.2.2 Facilities/equipment; 4.3 Methods; 4.3.1 On chip polyacrylamide gel electrophoresis (PAGE); 4.3.2 Polyacrylamide gel electrophoresis based isoelectric focusing; 4.3.3 Data acquisition, anticipated results, and interpretation; 4.3.4 Results and discussion; 4.4 Discussion of pitfalls; 4.5 Summary notes. 
520 |a This unique volume presents leading-edge microfluidics methods used to handle, manipulate, and analyze cells, particles, and biological components (e.g., proteins and DNA) for microdiagnostics. The authors offer clear and detailed guidance on microfabrication techniques utilized to create microfluidic devices and on-chip flow control and mixing Microsystems, protein and DNA handling devices for electrophoretic and isoelectric separations in microchromatography columns, microfluidic manipulations of droplets via electrowetting and particles via dielectrophoresis for separations and chemical rea. 
588 0 |a Print version record. 
650 0 |a Bioengineering  |x Methodology. 
650 0 |a Microfabrication. 
650 0 |a Microfluidics. 
650 7 |a Microfabrication  |2 fast 
650 7 |a Microfluidics  |2 fast 
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830 0 |a Methods in bioengineering. 
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