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Synthetic Biology :
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Synthetic Biology : Parts, Devices and Applications.
Saved in:
Bibliographic Details
Main Author:
Lee, Sang Yup
Other Authors:
Nielsen, Jens
,
Stephanopoulos, Gregory
,
Smolke, Christina
Format:
eBook
Language:
English
Published:
Newark :
John Wiley & Sons, Incorporated,
2018.
Series:
Advanced Biotechnology Ser.
Subjects:
Synthetic biology.
Synthetic biology
Online Access:
Click for online access
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Description
Table of Contents
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Table of Contents:
Cover; Title Page; Copyright; Contents; About the Series Editors; Part I DNA Synthesis and Genome Engineering; Chapter 1 Competition and the Future of Reading and Writing DNA; 1.1 Productivity Improvements in Biological Technologies; 1.2 The Origin of Mooreâ#x80;#x99;s Law and Its Implications for Biological Technologies; 1.3 Lessons from Other Technologies; 1.4 Pricing Improvements in Biological Technologies; 1.5 Prospects for New Assembly Technologies; 1.6 Beyond Programming Genetic Instruction Sets; 1.7 Future Prospects; References.
Chapter 2 Trackable Multiplex Recombineering (TRMR) and Next-Generation Genome Design Technologies: Modifying Gene Expression in E. coli by Inserting Synthetic DNA Cassettes and Molecular Barcodes2.1 Introduction; 2.2 Current Recombineering Techniques; 2.2.1 Recombineering Systems; 2.2.2 Current Model of Recombination; 2.3 Trackable Multiplex Recombineering; 2.3.1 TRMR and T2RMR Library Design and Construction; 2.3.2 Experimental Procedure; 2.3.3 Analysis of Results; 2.4 Current Challenges; 2.4.1 TRMR and T2RMR are Currently Not Recursive; 2.4.2 Need for More Predictable Models.
2.5 Complementing Technologies2.5.1 MAGE; 2.5.2 CREATE; 2.6 Conclusions; Definitions; References; Chapter 3 Site-Directed Genome Modification with Engineered Zinc Finger Proteins; 3.1 Introduction to Zinc Finger DNA-Binding Domains and Cellular Repair Mechanisms; 3.1.2 Homologous Recombination; 3.1.3 Non-homologous End Joining; 3.2 Approaches for Engineering or Acquiring Zinc Finger Proteins; 3.2.1 Modular Assembly; 3.2.2 OPEN and CoDA Selection Systems; 3.2.3 Purchase via Commercial Avenues; 3.3 Genome Modification with Zinc Finger Nucleases.
3.4 Validating Zinc Finger Nuclease-Induced Genome Alteration and Specificity3.5 Methods for Delivering Engineered Zinc Finger Nucleases into Cells; 3.6 Zinc Finger Fusions to Transposases and Recombinases; 3.7 Conclusions; References; Chapter 4 Rational Efforts to Streamline the Escherichia coli Genome; 4.1 Introduction; 4.2 The Concept of a Streamlined Chassis; 4.3 The E. coli Genome; 4.4 Random versus Targeted Streamlining; 4.5 Selecting Deletion Targets; 4.5.1 General Considerations; 4.5.1.1 Naturally Evolved Minimal Genomes; 4.5.1.2 Gene Essentiality Studies.
4.5.1.3 Comparative Genomics4.5.1.4 In silico Models; 4.5.1.5 Architectural Studies; 4.5.2 Primary Deletion Targets; 4.5.2.1 Prophages; 4.5.2.2 Insertion Sequences (ISs); 4.5.2.3 Defense Systems; 4.5.2.4 Genes of Unknown and Exotic Functions; 4.5.2.5 Repeat Sequences; 4.5.2.6 Virulence Factors and Surface Structures; 4.5.2.7 Genetic Diversity-Generating Factors; 4.5.2.8 Redundant and Overlapping Functions; 4.6 Targeted Deletion Techniques; 4.6.1 General Considerations; 4.6.2 Basic Methods and Strategies; 4.6.2.1 Circular DNA-Based Method; 4.6.2.2 Linear DNA-Based Method.
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