The Metabolic Pathway Engineering Handbook : tools and applications

The Metabolic Pathway Engineering Handbook : tools and applications / edited by Christina D. Smolke.

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Bibliographic Details
Other Authors: Smolke, Christina D. (Editor)
Format: eBook
Language:English
Published: Boca Raton, FL : CRC Press, 2009.
Subjects:
Genetic engineering > Handbooks, manuals, etc.
Biosynthesis > Handbooks, manuals, etc.
SCIENCE > Chemistry > Industrial & Technical.
TECHNOLOGY & ENGINEERING > Chemical & Biochemical.
Biosynthesis
Genetic engineering
Handbooks and manuals
Online Access:Click for online access
Table of Contents:
  • Cover; Volume I; Half Title; Title Page; Copyright Page; Contents; Introduction; Editor; Contirbutors; SECTION I: Evolutionary Tools in Metabolic Engineering; 1 Evolutionary Engineering of Industrially Important Microbial Phenotypes; 2 Improving Protein Functions by Directed Evolution; 3 Engineering DNA and RNA Regulatory Regions through Random Mutagenesis and Screening; 4 Evolving Pathways and Genomes for the Production of Natural and Novel Compounds; 5 Models Predicting Optimized Strategies for Protein Evolution; SECTION II: Gene Expression Tools for Metabolic Pathway Engineering
  • 6 Low-Copy Number Plasmids as Artificial Chromosomes7 Chromosomal Engineering Strategies; 8 Regulating Gene Expression through Engineered RNA Technologies; 9 Tools Designed to Regulate Translational Efficiency; 10 Metabolic Engineering of the Secretory Processing Pathway in Eukaryotes; 11 Engineering Multifunctional Enzyme Systems for Optimized Metabolite Transfer between Sequential Conversion Steps; 12 Practical Pathway Engineering-Demonstration in Integrated Tools; SECTION III: Application of Emerging Technologies to Metabolic Engineering
  • 13 Genome-Wide Technologies: DNA Microarrays, Phenotypic Microarrays, and Proteomics14 Monitoring and Measuring the Metabolome; SECTION IV: Future Prospects in Metaboli Engineering; 15 Systems Biology, Genome-Scale Models, and Metabolic Engineering; 16 Cell-Free Systems for Metabolic Engineering; 17 In Silico Models for Metabolic Systems Engineering; SECTION V: Tools for Experimentally Determining Flux through Pathways; 18 GC-MS for Metabolic Flux Analysis; 19 Tools for Measuring Intermediate and Product Formation
  • 20 Use of AEX-HPLC-ESI-MS for [sup(13)]C-Labeling Based Metabolic Flux Analysis in Saccharomyces cerevisiae and Penicillium chrysogenumSECTION VI: Future Applicationf of Meabolic Engineering; 21 Energy and Cofactor Issues in Fermentation and Oxyfunctionalization Processes; 22 Microbial Biosynthesis of Fine Chemicals: An Emerging Technology; 23 Applications of Metabolic Engineering for Natural Drug Discovery; 24 Metabolic Engineering for Alternative Fuels; Volume II; Half Title; Title Page; Copyright Page; Contents; Introduction; Editor; Contributors; SECTION I: Cellular Metabolism
  • 1 Solute Transport Processes in the Cell2 Catabolism and Metabolic Fueling Processes; 3 Biosynthesis of Cellular Building Blocks: The Prerequisites of Life; 4 Polymerization of Buliding Blocks to Macromolecules Polyhydroxyalkanoates as an Example; 5 Rare Metabolic Conversions-Harvesting Diversity through Nature; SECTION II: Balances and Reaction Models; 6 Growth Nutrients and Diversity; 7 Mass Balances, Rates, and Experiments; 8 Data Reconciliation and Error Detection; 9 Black Box Models for Growth and Product Formation; 10 Metabolic Models for Growth and Product Formation

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