Arabidopsis BUPS-ANX receptor complex coordinates with RALF peptides to regulate pollen tube integrity and sperm release

Arabidopsis BUPS-ANX receptor complex coordinates with RALF peptides to regulate pollen tube integrity and sperm release / Zengxiang Ge.

This book summarizes the latest studies on plant reproduction and multiple aspects of signaling in reproductive development. It also presents the most advanced processes in CrRLK1L receptor and RALF peptide studies during plant development. Focusing on signaling in pollen tube integrity and sperm re...

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Bibliographic Details
Main Author: Ge, Zengxiang
Format: eBook
Language:English
Published: Singapore : Springer, 2020.
Series:Springer theses.
Subjects:
Pollen tube.
Cellular biology (cytology)
Proteins.
Plant physiology.
Science > Life Sciences > Biology > Molecular Biology.
Science > Life Sciences > Biochemistry.
Science > Life Sciences > Botany.
Pollen tube
Reproducció de les plantes.
Genètica vegetal.
Llibres electrònics.
Online Access:Click for online access
Table of Contents:
  • Intro
  • Supervisor's Foreword
  • Abstract
  • Parts of this book have been published in the following articles:Ge, Z., Bergonci, T., Zhao, Y.L., Zou, Y.J., Du, S., Liu, M.C., Luo, X.J., Ruan, H., Garcia-Valencia, L.E., Zhong, S., et al. (2017). Arabidopsis pollen tube integrity and sperm release are regulated by RALF-mediated signaling. Science 358, 1596-1599. Ge, Z., Cheung, A.Y., and Qu, L-J. (2019). Pollen tube integrity regulation in flowering plants: insights from molecular assemblies on the pollen tube surface. New Phytol. 222, 687-693
  • Acknowledgments
  • Contents
  • Abbreviations
  • 1 Review of Cell-Cell Communication in Plant Reproduction
  • 1.1 Overview of Plant Reproduction
  • 1.1.1 Interaction Between Pollen and the Stigma
  • 1.1.2 Pollen Tube Growth in the Transmitting Tract
  • 1.1.3 Pollen Tube Guidance
  • 1.1.4 Pollen Tube Reception
  • 1.1.5 Male and Female Gametophyte Fusion
  • 1.2 Plant Peptides and RLKs-Mediated Signaling in Plant Fertilization
  • 1.3 Summary and Perspective
  • References
  • 2 Receptor-Like Kinases BUPS1/2 are Involved in Pollen Tubes Integrity Maintenance in Arabidopsis
  • 2.1 Introduction
  • 2.2 Materials and Methods
  • 2.2.1 Plant Material
  • 2.2.2 Strains and Plasmids
  • 2.2.3 Chemicals and Reagents
  • 2.2.4 Methods
  • 2.3 Results
  • 2.3.1 Expression Pattern and Subcellular Localization of BUPS1/2
  • 2.3.2 Using CRISPR/Cas9 Technology to Knock Out BUPS1/2
  • 2.3.3 bups1 and bups1 bups2 Mutant Show Fertility Defect
  • 2.3.4 bups1 and bups1 bups2 are Male Sterile
  • 2.3.5 Pollen Development of bups1 or bups1 bups2 is Normal
  • 2.3.6 The Pollen Tube of bups1 and bups1 bups2 Rupture Prematurely
  • 2.3.7 BUPS1/2 Interact with ANX1/2 to Form a Receptor Complex
  • 2.4 Discussion
  • References
  • 3 RALF4/19 are Autocrine Signals to Maintain Pollen Tubes Integrity
  • 3.1 Introduction
  • 3.2 Materials and Methods
  • 3.2.1 Plant Material
  • 3.2.2 Strains and Plasmids
  • 3.2.3 Chemicals and Reagents
  • 3.2.4 Methods
  • 3.3 Results
  • 3.3.1 Expression Pattern and Subcellular Localization of RALF4/19
  • 3.3.2 Using CRISPR/Cas9 Technology to Knock Out RALF4/19
  • 3.3.3 ralf4 ralf19 Double Mutants are Male Sterile
  • 3.3.4 ralf4 ralf19 Exhibits bups1 bups2-like Phenotypes
  • 3.3.5 RALF4/19 Interact with BUPS1/2 Ectodomains
  • 3.3.6 RALF4/19 Show High Affinity to ANX1/2
  • 3.4 Discussion

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