Engineering of microbial biosynthetic pathways

Engineering of microbial biosynthetic pathways / Vijai Singh, Ajay Kumar Singh, Poonam Bhargava, Madhvi Joshi, Chaitanya G. Joshi, editors.

This book provides a comprehensive overview of the basic and advanced metabolic engineering technologies used to generate natural metabolites and industrially important biomolecules. Metabolic engineering has the potential to produce large quantities of valuable biomolecules in a renewable and susta...

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Bibliographic Details
Other Authors: Singh, Vijai (Editor), Singh, Ajay Kumar (Professor of food process engineering) (Editor), Bhargava, Poonam (Editor), Joshi, Madhvi (Editor), Joshi, Chaitanya G. (Editor)
Format: eBook
Language:English
Published: Singapore : Springer, 2020.
Subjects:
Microbial biotechnology.
Biotechnological microorganisms.
Genetic engineering.
Microbiology (non-medical)
Biochemical engineering.
Bio-ethics.
Computer modelling & simulation.
Biomedical engineering.
Science > Life Sciences > Genetics & Genomics.
Science > Life Sciences > Biology > Microbiology.
Technology & Engineering > Chemical & Biochemical.
Philosophy > Ethics & Moral Philosophy.
Computers > Computer Simulation.
Medical > Allied Health Services > Medical Technology.
Biotechnological microorganisms
Microbial biotechnology
Online Access:Click for online access
Table of Contents:
  • Intro
  • Foreword
  • Preface
  • Acknowledgments
  • Contents
  • About the Editors
  • 1: An Introduction to Design of Microbial Strain using Synthetic Biology Toolboxes for Production of Biomolecules
  • 1.1 Introduction
  • 1.2 Desired Characteristics of Strain Used for Production of Biomolecules
  • 1.3 Methods Used for Strain Improvement
  • 1.4 Protein Engineering
  • 1.5 Directed Evolution
  • 1.6 Design and Construction of Biosynthetic Pathways
  • 1.7 Synthetic Biology Toolbox in Metabolic Engineering
  • 1.8 Adaptive Laboratory Evolution
  • 1.9 Conclusion and Future Remarks
  • References
  • 2: Microbial Strain Engineering
  • 2.1 Introduction
  • 2.1.1 Need and Significance for Strain Improvement
  • 2.2 Mutagenesis
  • 2.2.1 Physical and Chemical Mutagenesis
  • 2.2.2 Mutation Signature
  • 2.3 Engineering Physiology of Microbes
  • 2.3.1 Desired Physiological Characteristics
  • 2.3.2 Candidate Screening
  • 2.3.3 Selection of Host Strain
  • 2.3.4 Engineering into the Host Strain
  • 2.4 Metabolic Engineering
  • 2.4.1 Methodologies and Tools for Metabolic Engineering
  • 2.4.2 Engineering of Biosynthetic Pathways
  • 2.4.3 Central Metabolism Engineering
  • 2.4.4 Transport Engineering
  • 2.4.5 Engineering the Whole Cell
  • 2.5 Genetic Engineering
  • 2.5.1 Screening
  • 2.5.2 Gene Expression
  • 2.5.3 Enzyme (Over)Production and Posttranscriptional Control
  • 2.6 Omics for Strain Engineering
  • 2.6.1 Genome Analysis
  • 2.6.2 Transcriptome Analysis
  • 2.6.3 Proteome Analysis
  • 2.6.4 Fluxome and Metabolome Analysis
  • 2.6.4.1 Metabolome Analysis
  • 2.6.4.2 Fluxome Analysis
  • 2.6.5 Combined Omics Approach
  • 2.7 Conclusion
  • References
  • 3: Techniques for Detection and Extraction of Metabolites
  • 3.1 Introduction
  • 3.2 Extraction of Metabolites
  • 3.2.1 Quenching Methods
  • 3.2.1.1 Perchloric Acid
  • 3.2.1.2 Liquid Nitrogen Method
  • 3.2.1.3 Methanol Method
  • 3.2.2 Extraction of Extracellular Metabolites
  • 3.2.3 Extraction of Intracellular Metabolites
  • 3.2.3.1 Non-mechanical Lysis
  • Boiling Ethanol
  • Cold Methanol
  • Buffered Methanol-Chloroform-Water
  • Hot Water
  • 3.2.3.2 Mechanical Lysis of Cell
  • Supercritical Fluid Extraction
  • Pressurized Liquid Extraction
  • 3.3 Detection of Metabolites
  • 3.3.1 Mass-Spectrometry (MS)
  • 3.3.2 Gas Chromatography-MS
  • 3.3.3 Capillary Electrophoresis-MS (CE-MS)
  • 3.3.4 Liquid Chromatography-MS (LC-MS)
  • 3.3.5 Nuclear Magnetic Resonance
  • 3.4 Conclusion
  • References
  • 4: Genetically Encoded Biosensors and Their Applications in the Development of Microbial Cell Factories
  • 4.1 The Classification of Genetically Encoded Biosensors
  • 4.1.1 Protein-Based Biosensors
  • 4.1.1.1 The Functional Mechanism of Protein-Based Biosensors
  • 4.1.1.2 Designing and Tuning Protein-Based Biosensors
  • Tuning at aTF Level
  • Tuning at Promoter Level
  • 4.1.2 RNA-Based Biosensors
  • 4.1.2.1 The Functional Mechanism of RNA-Based Biosensors
  • 4.1.2.2 Designing and Tuning RNA-Based Biosensors

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